Effects of low-level laser therapy on ROS homeostasis and expression of IGF-1 and TGF-β1 in skeletal muscle during the repair process
Wed, 2012-06-20 07:54 — Guest
Abstract
The aim of the present study was to determine the effects of low-level laser therapy (LLLT) on the homeostasis of reactive
oxygen species (ROS) and expression of IGF-1 and TGF-β1 in the gastrocnemius muscles of rats following contusion. Muscle regeneration
involves cell proliferation, migration, and differentiation and is regulated by growth factors. A growing body of evidence
suggests that LLLT promotes skeletal muscle regeneration and accelerates tissue repair. Adult male Sprague-Dawley rats (n = 96) were randomly divided into three groups: control group (no lesion, untreated, n = 6), contusion group (n = 48), and contusion-plus-LLLT group (n = 42). Gallium aluminum arsenide (GaAlAs) laser irradiation (635 nm; beam spot, 0.4 cm2; output power, 7 mW; power density, 17.5 mW/cm2; 20 min) was administered to the gastrocnemius contusion for 20 min daily for 10 days. Muscle remodeling was evaluated at
0 h and 1, 2, 3, 7, 14, 21, and 28 days after injury. Hematoxylin and eosin and Van Gieson staining were used to evaluate
regeneration and fibrosis; muscle superoxide dismutase (SOD) and malondialdehyde (MDA) were detected via biochemical methods;
expression of transforming growth factor beta-1 (TGF-β1) and insulin-like growth factor-1 (IGF-1) were investigated via immunohistochemistry.
The results showed that LLLT markedly promoted the regeneration of muscle and reduced scar formation. LLLT also significantly
enhanced muscle SOD activity and significantly decreased muscle MDA levels 1, 2, and 3 days after injury. LLLT increased the
expression of IGF-1 2, 3, and 7 days after injury and decreased the expression of IGF-1 21 and 28 days after injury. LLLT
decreased the expression of TGF-β1 3 and 28 days after injury but increased expression at 7 and 14 days after injury. Our
study showed that LLLT could modulate the homeostasis of ROS and of the growth factors IGF-1 and TGF-β1, which are known to
play important roles in the repair process. This may constitute a new preventive approach to muscular fibrosis.
oxygen species (ROS) and expression of IGF-1 and TGF-β1 in the gastrocnemius muscles of rats following contusion. Muscle regeneration
involves cell proliferation, migration, and differentiation and is regulated by growth factors. A growing body of evidence
suggests that LLLT promotes skeletal muscle regeneration and accelerates tissue repair. Adult male Sprague-Dawley rats (n = 96) were randomly divided into three groups: control group (no lesion, untreated, n = 6), contusion group (n = 48), and contusion-plus-LLLT group (n = 42). Gallium aluminum arsenide (GaAlAs) laser irradiation (635 nm; beam spot, 0.4 cm2; output power, 7 mW; power density, 17.5 mW/cm2; 20 min) was administered to the gastrocnemius contusion for 20 min daily for 10 days. Muscle remodeling was evaluated at
0 h and 1, 2, 3, 7, 14, 21, and 28 days after injury. Hematoxylin and eosin and Van Gieson staining were used to evaluate
regeneration and fibrosis; muscle superoxide dismutase (SOD) and malondialdehyde (MDA) were detected via biochemical methods;
expression of transforming growth factor beta-1 (TGF-β1) and insulin-like growth factor-1 (IGF-1) were investigated via immunohistochemistry.
The results showed that LLLT markedly promoted the regeneration of muscle and reduced scar formation. LLLT also significantly
enhanced muscle SOD activity and significantly decreased muscle MDA levels 1, 2, and 3 days after injury. LLLT increased the
expression of IGF-1 2, 3, and 7 days after injury and decreased the expression of IGF-1 21 and 28 days after injury. LLLT
decreased the expression of TGF-β1 3 and 28 days after injury but increased expression at 7 and 14 days after injury. Our
study showed that LLLT could modulate the homeostasis of ROS and of the growth factors IGF-1 and TGF-β1, which are known to
play important roles in the repair process. This may constitute a new preventive approach to muscular fibrosis.
- Content Type Journal Article
- Category Original Article
- Pages 1-10
- DOI 10.1007/s10103-012-1133-0
- Authors
- Li Luo, School of Physical Education and Sports Science, Soochow University, Suzhou, 215021 China
- Zhongwen Sun, Department of Microbiology and Immunology, Suzhou Health College of Vocational Technology, Suzhou, 215104 China
- Lin Zhang, School of Physical Education and Sports Science, Soochow University, Suzhou, 215021 China
- Xiaoning Li, Department of Pathology, Suzhou Health College of Vocational Technology, Suzhou, 215104 China
- Yu Dong, Department of Orthopaedics and Sports Medicine, Huashan Hospital, Fudan University, Shanghai, 200040 China
- Timon Cheng-Yi Liu, College of Sports Science and Research Center of Nationalistic Constitution and Health, South China Normal University, Guangzhou, 510006 China
- Journal Lasers in Medical Science
- Online ISSN 1435-604X
- Print ISSN 0268-8921
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